calbindin  (Jackson Immuno)

Journal: Neural Regeneration Research

Article Title: Tracing motor neurons and primary sensory afferents of the monkey spinal cord with cholera toxin subunit B

doi: 10.4103/NRR.NRR-D-24-00995

Figure Lengend Snippet: CTB-labeled primary sensory afferents form synapses with multiple types of monkey spinal neurons. (A) Representative images of CTB, Syn and Homer1 immunostaining showing that CTB + terminals expressed the presynaptic marker Syn and were in close proximity to the postsynaptic marker Homer1. (B, C) Magnified images from A confirming that the CTB + terminus expressed synaptophysin and was in close proximity to Homer1. (D–I) CTB + VGLUT1 + terminals formed synapses with LMX1B + (D), LHX1 + (E), calretinin + (F), calbindin + (G), FOXP2 + (H) and SATB1 + (I) neurons. (J) Image of the ventral horn showing two motor neurons, one labeled with CTB and the other not labeled with CTB. (K) Magnified images from J showing that CTB + VGLUT1 + terminals formed synapses with motor neurons. (L) The CTB + terminus was innervated by GAD65 + inhibitory synapses. Scale bars: 20 μm in J and K; 5 μm in A, D–I, and L; and 1 μm in B and C. CTB: Cholera toxin subunit B; FOXP2: forkhead box protein P2; GAD65: glutamic acid decarboxylase 65; LHX1: LIM homeobox 1; LMX1B: LIM homeobox transcription factor 1 beta; SATB1: special AT-rich sequence-binding protein 1; Syn: synaptophysin; VGLUT1: vesicular glutamate transporter 1.

Article Snippet: Calbindin , Rabbit/1:1000 , Swant , CB38/AB_10000340.

Techniques: Labeling, Immunostaining, Marker, Sequencing, Binding Assay

Journal: PLOS One

Article Title: Impaired dynein function preserves spinal interneuron survival and positioning in an ALS-like mouse model

doi: 10.1371/journal.pone.0346246

Figure Lengend Snippet: Number of ChAT + (A-C), GAD-67 + (D), Calbindin + (E), Parvalbumin + (F), Parvalbumin + −Calbindin + (G), Parvalbumin + −GAD-67 + (H), and ChAT + −GAD-67 + (I) cells per biological replicate. The number of cells analysed is the sum of immunostained positive cells from eight spinal cord cross-sections (two sections per lumbar level L3–L6) per biological replicate. Data are shown as mean ± standard deviation and were analysed by an ordinary one-way ANOVA. N = 3 biological replicates per genotype.

Article Snippet: Following three 10-min PBS washes at 100 rpm, secondary probing was performed for 3 hours in the dark in PBS containing 0.05% Tween-20 and the relevant secondary antibodies for ChAT (1:250 dilution, donkey anti-goat IgG H + L Alexa Fluor ® 405, Abcam, ab175664), Calbindin (1:500 dilution, Rhodamine TRITC-AffiniPure donkey anti-guinea pig Ig G H + L, Jackson Immuno Research, 706-025-148-JIR), Parvalbumin (1:500 dilution, Alexa Fluor 647-AffiniPure donkey anti-rabbit IgG H + L, Jackson Immuno Research, 711-605-152), and GAD-67 (1:500 dilution, donkey anti-chicken IgY H + L Highly Cross Adsorbed Alexa Fluor ® 488, Life Technologies, A78948).

Techniques: Standard Deviation